Sodium influxes in internally perfused squid giant axon during voltage clamp
نویسندگان
چکیده
منابع مشابه
Stoichiometry and voltage dependence of the sodium pump in voltage- clamped, internally dialyzed squid giant axon
The stoichiometry and voltage dependence of the Na/K pump were studied in internally dialyzed, voltage-clamped squid giant axons by simultaneously measuring, at various membrane potentials, the changes in Na efflux (delta phi Na) and holding current (delta I) induced by dihydrodigitoxigenin (H2DTG). H2DTG stops the Na/K pump without directly affecting other current pathways: (a) it causes no de...
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Probably the most striking and surprising observation in internally perfused axons is that full-sized action potentials can be obtained in the face of a very low resting potential when the ionic strength is low In all experiments at normal ionic strength known to the author, the explosive all-or-none responsiveness was lost when the internal and/or external monovalent cations were arranged to g...
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The sodium conductance of the membrane of the giant axon of squid was isolated by the use of potassium-free solutions and voltage-clamped with pulses containing three levels of depolarization. The conductance appears to undergo rapid changes during certain repolarizing clamp steps whose voltage reach at least partially overlaps the gating range. The percentage change in conductance increases wi...
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Sodium movements in internally perfused giant axons from the squid Dosidicus gigas were studied with varying internal sodium concentrations and with fluoride as the internal anion. It was found that as the internal concentration of sodium was increased from 2 to 200 mM the resting sodium efflux increased from 0.09 to 34.0 pmoles/cm(2)sec and the average resting sodium influx increased from 42.9...
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Isolated and cleaned giant axons of Loligo pealii were internally perfused with solutions containing cesium sulfate and potassium fluoride. Membrane currents obtained as a function of clamped membrane potentials indicated a severe depression of the delayed outward current component normally attributed to potassium ion movement. Steady-state currents showed a negative slope in the potential rang...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1969
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1969.sp008778